the correlation between telomerase activity and bax/bcl-2 ratio in valproic acid-treated mcf-7 breast cancer cell line

Authors

zahra vafaiyan department of biochemistry and biophysics, disorder metabolic research center, golestan university of medical sciences, gorgan, iran

roghaye gharaei department of biochemistry and biophysics, disorder metabolic research center, golestan university of medical sciences, gorgan, iran

jahanbakhsh asadi department of biochemistry and biophysics, disorder metabolic research center, golestan university of medical sciences, gorgan, iran

abstract

objective(s):valproic acid (vpa), a drug used in the treatment of neurological disorders, has been shown to have cytotoxic effects on cancer cells through different mechanisms. telomerase, a ribonucleoprotein reverse transcriptase, is responsible for elongation of the telomere and is activated in cancers. a relation between telomerase activity and resistance to apoptosis has been established. this study focused on probable effects of vpa on mcf-7 cancer cells. in particular, we investigated vpa effects on viability, apoptosis and telomerase activity. materials and methods: cytotoxicity effects of vpa on mcf-7 cells were determined by neutral red uptake assay. cells were treated with different concentrations of vpa (0-32 mm) and telomerase activity and bax and bcl-2 protein levels were determined using trap assay (pcr-elisa) method and elisa method, respectively. results: the cytotoxic effects of different concentration of vpa on mcf-7 cells were observed as a reduction in cell viability and telomerase activity and altered expression of bcl-2 family protein levels. the results also showed that there is a significant correlation between reduction of telomerase activity and increase in bax/bcl-2 ratio (p=0.001). conclusion: our study demonstrated that cell viability of mcf-7 cells was decreased after treatment with vpa, probably through a reduction of telomerase activity and an increase in bax/bcl-2 ratio. therefore, it could be concluded that vpa is a potent anti-cancer agent for breast cancer cells through inhibition of telomerase activity and induction of apoptosis.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

The correlation between telomerase activity and Bax/Bcl-2 ratio in valproic acid-treated MCF-7 breast cancer cell line

Objective(s):Valproic acid (VPA), a drug used in the treatment of neurological disorders, has been shown to have cytotoxic effects on cancer cells through different mechanisms. Telomerase, a ribonucleoprotein reverse transcriptase, is responsible for elongation of the telomere and is activated in cancers. A relation between telomerase activity and resistance to apoptosis has been established. T...

full text

The correlation between telomerase activity and Bax/Bcl-2 ratio in valproic acid-treated MCF-7 breast cancer cell line

OBJECTIVES Valproic acid (VPA), a drug used in the treatment of neurological disorders, has been shown to have cytotoxic effects on cancer cells through different mechanisms. Telomerase, a ribonucleoprotein reverse transcriptase, is responsible for elongation of the telomere and is activated in cancers. A relation between telomerase activity and resistance to apoptosis has been established. Thi...

full text

the simultaneous effect of valproic acid and gamma radiation on telomerase activity and bax and bcl-2 protein levels in mcf-7 breast cancer cell line

conclusions the increasing cell toxicity, apoptosis-inducing effects and decreasing telomerase activity may play an important role in the valproic acid and radiation mechanism. the current survey suggested that it is likely beneficial to combine valproic acid and gamma radiation to treat breast cancer. results combination of valproic acid and gamma radiation increased significantly cell toxicit...

full text

The Activity of Sirtuin 1 in MCF-7 Breast Cancer Cell Line: The Effects of Visfatin

Background & Objectives: Breast cancer is the most common cancer and the second leading cause of cancer deaths among women. Obesity, hormones, and growth factors are the risk factors for this kind of cancer. One of the changes observed in patients suffering from breast cancer is the elevated Visfatin or nicotinamide phosphoribosyl transferase (NAMPT) in their tumor tissues and blood. The increa...

full text

Investigating the antioxidant and anti-proliferative activity of Foeniculum vulgare seed essential oil on MCF-7 breast cancer cell line and A2780 ovarian cancer cell line

Background & aim: Due to the presence of high amounts of compounds with antioxidant and cytotoxic properties in Foeniculum vulgare mill seed essential oil, the aim of this study was to investigate the toxicity of Foeniculum vulgare mill seed essential oil on MCF-7 breast cancer cell line and A2780 ovarian cancer cell line and to investigate the antioxidant activity of the essential oil of this ...

full text

Cell Kinetic Study of Tamoxifen Treated MCF-7 and MDA-MB 468 Breast Cancer Cell Lines

Apoptosis could be a major mechanism of antitumor effect of tamoxifen. Therefore this study is designed to characterize the kinetic behavior of tamoxifen-induced apoptosis in the estrogen receptor positive (ER+) and negative (ER-) cell lines, MCF-7 and MDA-MB-468. Frequency of cell death was examined by trypan blue and acridine orange staining. Annexin V-Fluorescein/PI was used in flow cytometr...

full text

My Resources

Save resource for easier access later


Journal title:
iranian journal of basic medical sciences

جلد ۱۸، شماره ۷، صفحات ۷۰۰-۷۰۴

Keywords
[ ' b a x ' , ' b c l ' , 2 , ' m c f ' , 7 , ' t e l o m e r a s e ' , ' v a l p r o i c a c i d ' ]

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023